HMGB1 Knockout cell line （LS174T）

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

HMGB1 Knockout cell line （LS174T） Order

Catalog#

YKO-H913

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

HMGB1 Knockout cell line（LS 174T）

Morphology

Epithelial-like,adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

HMGB1

Gene ID

3146

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 3 (based on transcript HMGB1-203)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HMGB1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the LS 174T cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HMGB1 gene knockout HMGB1 Knockout cell line（LS 174T） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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